[IF=0] Sun, Weijia, et al. "Osteoclast-derived microRNA-containing exosomes selectively inhibit osteoblast activity." Cell Discovery 2 (2016).  WB ;  Mouse.  

PubMed:27462462

[IF=0] Wang CJ et al. Exosome?delivered TRPP2 siRNA inhibits the epithelial?mesenchymal transition of FaDu cells. Oncol Lett. 2019 Feb;17(2):1953-1961.   WB ;  Human.  

PubMed:30675260

[IF=9.598] Sung MS et al. Single-Molecule Co-Immunoprecipitation Reveals Functional Inheritance of EGFRs in Extracellular Vesicles. (2018) Small,14(42):e1802358.   FCM WB ;  

PubMed:30239124

[IF=5.58] Marzesco, Anne-Marie, et al. "Highly potent intracellular membrane-associated Aβ seeds." Scientific Reports 6 (2016): 28125.  WB, other ;  Mouse.  

PubMed:27311744

Sample:
A431 cell(human) Lysate at 40 ug
HL-60 cell(human) Lysate at 40 ug
MCF-7 cell(human) Lysate at 40 ug
U87mg cell(human)Lysate at 40 ug
Primary: Anti- CD63 (bs-1523R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26kD
Observed band size: 53 kD

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD63 Polyclonal Antibody, Unconjugated(bs-1523R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

cell: A549 cells
Incubation: Avoid light, at 4°C for 60 minutes.
Blank control A549 cells(red line),5X10^5/ml, at 4°C for 60 minutes.
primary antibody: abbit Anti-CD63 antibody (bs-1523R-PE,Blue line) ,1:50, at 4°C for 60 minutes.

Blank control: Hep G2 cells(blue).
Primary Antibody:Rabbit Anti- CD23 antibody(bs-1523R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (bs-1523R,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.